Overview of the experiment design

Plate phenotyping experiment:

Example experiment

Plate layout

The images will be interpreted like this, using plate001.jpg as an example

1 2 3 4 5 6
c,1,foo c,2,foo d,1,bar d,2,bar
c,3,foo c,4,foo c,5,foo d,3,bar d,4,bar d,5,bar
c,6,foo c,7,foo c,8,foo d,6,bar d,7,bar d,8,bar <- Mark
b,1,baz b,2,baz b,3,baz a,1,qux a,2,qux a,3,qux
b,4,baz b,5,baz b,6,baz a,4,qux a,5,qux a,6,qux
b,7,baz b,8,baz a,7,qux a,8,qux

and the plates should be sown like this using the same example

1 2 3 4 5 6
a,8,qux a,7,qux b,8,baz b,7,baz
a,6,qux a,5,qux a,4,qux b,6,baz b,5,baz b,4,baz
Mark -> a,3,qux a,2,qux a,1,qux b,3,baz b,2,baz b,1,baz
d,8,bar d,7,bar d,6,bar c,8,foo c,7,foo c,6,foo
d,5,bar d,4,bar d,3,bar c,5,foo c,4,foo c,3,foo
d,2,bar d,1,bar c,2,foo c,1,foo

Plate sowing

Sowing order is indicated below and also given as sowing-order.csv and in long format with one row per genotype in sowing-order-long.csv, and as short printable summary in sowing-summary.csv

plate label block position treatment genotypes regions
plate001 A1 1 1 control foo,bar,baz,qux c,d,b,a
plate004 A2 1 2 osmotic foo,bar,baz,qux c,d,b,a
plate006 B1 2 1 osmotic foo,bar,baz,qux c,d,b,a
plate002 B2 2 2 control foo,bar,baz,qux c,d,b,a
plate003 C1 3 1 control foo,bar,baz,qux c,d,b,a
plate005 C2 3 2 osmotic foo,bar,baz,qux c,d,b,a

A compact listing over which plates are to subjected to which treatment:

GENOTYPE genotype_region control osmotic
bar d 1 to 3 4 to 6
baz b 1 to 3 4 to 6
foo c 1 to 3 4 to 6
qux a 1 to 3 4 to 6

This report was compiled on Sat Jan 09 20:13:05 2016 by gbfjc on Windows using rosettR v0.0.2